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International Academy of Wood Science 2018 Annual Meeting

Papers Proceedings »

LIGNOCELULOSIC WASTE AS CARBON SOURCE FOR THE PRODUCTION OF BIOPOLIESTERS BY BACTERIA ISOLATED FROM FOREST SOIL

Agave bagasse was chemically hydrolyzed (catalyst, 2% sulfuric acid, particle size, 40 mesh, solids content, 10%, temperature, 130 °C, time, 20 min) to obtain fermentable sugars (xylose, 7.21 g/L; glucose, 3.09 g/L). Subsequently the hydrolyzate was treated with activated charcoal (50 g/L, agitation 4 h), to eliminate microbial growth inhibition compounds, centrifuged and neutralized (pH 7). This hydrolyzate was used for the production of biopolyesters by a bacterium belonging to the genus Achromobacter (isolated from a forest soil sample) in a two-stage process: 1. Biomass production for 48 h (hydrolyzate with addition of yeast extract and peptone); 2. Accumulation of the polymer during 48 h (recovery of biomass from stage 1 and inoculation in hydrolyzate without nitrogen source). Also, the microorganism was cultivated using xylose and glucose as a carbon source (10 g/L). Subsequently the bacterial biomass was recovered and the polymer was extracted and characterized by FTIR and NMR. The production of biomass (g/L) and accumulation of PHA (%) for each carbon source was: xylose (4.13 g/L, 31.3%), glucose (1.95 g/L, 16.9%), hydrolyzed agave (1.36 g/L, 8%). The spectra obtained by FTIR and NMR indicated that the bacterium synthesized from each carbon source polyhydroxybutyrate (PHB) which is an homopolymer of 3-hydroxybutyric acid.

Berenice Clifton García
Universidad de Guadalajara
Mexico

Yolanda González García
Universidad de Guadalajara
Mexico

Juan Villafaña Rojas
Universidad Autónoma de Guadalajara
Mexico

Orfil González Reynoso
Universidad de Guadalajara
Mexico

JORGE RAMON ROBLEDO ORTIZ
Universidad de Guadalajara
Mexico

 


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