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In vitro genotoxicity of extracts obtained from Eysenhardtia polystachya (Ort.) Sarg.
Currently, there is interest in bioactive substances, mainly from plants, because they show a chemical diversity such as phenolic, nitrogen and terpenoids compounds. These compounds are used for the treatment and cure of diseases. Signally, different plants used in traditional medicine does not have the scientific support, since there are no studies that provide the quality, safety and effectiveness of them, Even though they are commercialized putting health at risk. Eysenhardtia polystachya (Ort.) Sarg. (Palo dulce), belongs to the family Fabaceae, a tree widely distributed in Mexico and used in traditional medicine. The aim of the present work contributes to improve studies of genotoxicity since there is little information available on this topic. For this purpose, we evaluate the genotoxic activity in human lymphocytes of three fractions obtained from the extract of wood of E. polystachya, at three concentrations and at different exposure times, using the single cell gel electrophoresis, or comet assay, to measure the DNA damage of individual cells. A statistical analysis was carried out, comparing the different concentrations with respect to the negative control, being statistically significant (P <0.05) in the three fractions; methanolic fraction was what caused the best effect, with the greater damage independently of the dose and time of exposure, with 3.03±1.63 µ DNA migration.