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IDENTIFICATION OF WOODEN SPECIES THROUGH GENETIC MARKERS
Wood is considered a lignocellulosic bio-material which includes the following properties: macroscopic (smell, taste, color, texture), microscopic (vase element, fibers, parenchyma cells), physical (density, humidity, resistance to mechanical stress) and chemical (hemicelluloses, cellulose, lignin, etc.), these constituent elements provide diverse characteristics to each species, opening the possibility of their study. The identification of wood can be done from the tree, since it has the essential botanical elements: leaves, fruit, seeds, flowers, stem; however, when the wood is harvested or processed, it is difficult to identify it. In the present project the DNA extraction from leaf and wood of the five species: Pinus Pseudostrobus, Pinus ayacahuite, Liquidambar macrophylla, Cupressus arizonica, Pseudobombax septenatum was achieved following a protocol reported in the literature and later by PCR (polymerase chain reaction), achieving the amplification of their genes, with the oligonucleotides trnL and trnLF, determining the genetic markers for their amplification. By means of electrophoresis, 700 to 1200 pairs of bases were observed, which will be sequenced for implementation within the bar code called C.U.F.R.A, generating a system of identification and registration of tree species for the different sectors of timber interest that require it.